Neutralization:Article Title: Targeted Hyaluronan Degradation Enhanced Tumor Growth Inhibition in Gastrointestinal Cancer Models
Article Snippet: For the purification of antibodies and PH20-Fc, the filtered supernatants were loaded onto a MabSelect SuRe column (Cytiva, #11003494, Marlborough, MA, USA) using an AKTA pure 25 system (Cytiva, #29018224). .. The column was washed with PBS, and the bound antibodies were eluted using an elution buffer containing 150 mM sodium citrate, pH 3.4 (prepared in-house) and collected in tubes with 1 M tris-HCl solution, pH 9.0 (Sangon Biotech, #8548128, Shanghai, China) for rapid neutralization. .. For the purification of PH20-His6 protein, the supernatant was incubated with TALON Metal Affinity Resin (Clontech, #635501, Mountain View, CA, USA) for 2 h at 4 °C.
Molecular Weight:Article Title: A toehold-triggered switchable three-way junction protective nanoprobe for RNase H-assisted HBV rcDNA detection
Article Snippet: Bovine serum albumin (BSA) was obtained from Merck Chemical Technology Co., Ltd. Human serum albumin (HSA) was supplied by Nanjing Fengbo Biotechnology Co., Ltd. Magnesium chloride (MgCl 2 ), Tris(hydroxymethyl)aminomethane (Tris), boric acid (H 3 BO 3 ), disodium ethylenediaminetetraacetate dihydrate (Na 2 EDTA·2H 2 O), magnesium acetate tetrahydrate (Mg(CH 3 COO) 2 ·4H 2 O), and sodium chloride (NaCl) were all purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. .. Ammonium persulfate (APS), N,N, N’,N’-tetramethylethylenediamine (TEMED), Tris-HCl solution, diethyl pyrocarbonate (DEPC)-treated water, 30% acrylamide/bis-acrylamide solution (19:1), DNA molecular weight marker, and 4 S Gel-Red nucleic acid stain were obtained from Sangon Biotech (Shanghai) Co., Ltd. All other chemical reagents were of analytical grade and used without further purification. .. Ultrapure water (> 18 MΩ·cm) obtained from a Milipore water purification system was used for all solution preparations.
Marker:Article Title: A toehold-triggered switchable three-way junction protective nanoprobe for RNase H-assisted HBV rcDNA detection
Article Snippet: Bovine serum albumin (BSA) was obtained from Merck Chemical Technology Co., Ltd. Human serum albumin (HSA) was supplied by Nanjing Fengbo Biotechnology Co., Ltd. Magnesium chloride (MgCl 2 ), Tris(hydroxymethyl)aminomethane (Tris), boric acid (H 3 BO 3 ), disodium ethylenediaminetetraacetate dihydrate (Na 2 EDTA·2H 2 O), magnesium acetate tetrahydrate (Mg(CH 3 COO) 2 ·4H 2 O), and sodium chloride (NaCl) were all purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. .. Ammonium persulfate (APS), N,N, N’,N’-tetramethylethylenediamine (TEMED), Tris-HCl solution, diethyl pyrocarbonate (DEPC)-treated water, 30% acrylamide/bis-acrylamide solution (19:1), DNA molecular weight marker, and 4 S Gel-Red nucleic acid stain were obtained from Sangon Biotech (Shanghai) Co., Ltd. All other chemical reagents were of analytical grade and used without further purification. .. Ultrapure water (> 18 MΩ·cm) obtained from a Milipore water purification system was used for all solution preparations.
Staining:Article Title: A toehold-triggered switchable three-way junction protective nanoprobe for RNase H-assisted HBV rcDNA detection
Article Snippet: Bovine serum albumin (BSA) was obtained from Merck Chemical Technology Co., Ltd. Human serum albumin (HSA) was supplied by Nanjing Fengbo Biotechnology Co., Ltd. Magnesium chloride (MgCl 2 ), Tris(hydroxymethyl)aminomethane (Tris), boric acid (H 3 BO 3 ), disodium ethylenediaminetetraacetate dihydrate (Na 2 EDTA·2H 2 O), magnesium acetate tetrahydrate (Mg(CH 3 COO) 2 ·4H 2 O), and sodium chloride (NaCl) were all purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. .. Ammonium persulfate (APS), N,N, N’,N’-tetramethylethylenediamine (TEMED), Tris-HCl solution, diethyl pyrocarbonate (DEPC)-treated water, 30% acrylamide/bis-acrylamide solution (19:1), DNA molecular weight marker, and 4 S Gel-Red nucleic acid stain were obtained from Sangon Biotech (Shanghai) Co., Ltd. All other chemical reagents were of analytical grade and used without further purification. .. Ultrapure water (> 18 MΩ·cm) obtained from a Milipore water purification system was used for all solution preparations.
Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
Purification:Article Title: A toehold-triggered switchable three-way junction protective nanoprobe for RNase H-assisted HBV rcDNA detection
Article Snippet: Bovine serum albumin (BSA) was obtained from Merck Chemical Technology Co., Ltd. Human serum albumin (HSA) was supplied by Nanjing Fengbo Biotechnology Co., Ltd. Magnesium chloride (MgCl 2 ), Tris(hydroxymethyl)aminomethane (Tris), boric acid (H 3 BO 3 ), disodium ethylenediaminetetraacetate dihydrate (Na 2 EDTA·2H 2 O), magnesium acetate tetrahydrate (Mg(CH 3 COO) 2 ·4H 2 O), and sodium chloride (NaCl) were all purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. .. Ammonium persulfate (APS), N,N, N’,N’-tetramethylethylenediamine (TEMED), Tris-HCl solution, diethyl pyrocarbonate (DEPC)-treated water, 30% acrylamide/bis-acrylamide solution (19:1), DNA molecular weight marker, and 4 S Gel-Red nucleic acid stain were obtained from Sangon Biotech (Shanghai) Co., Ltd. All other chemical reagents were of analytical grade and used without further purification. .. Ultrapure water (> 18 MΩ·cm) obtained from a Milipore water purification system was used for all solution preparations.
Article Title: A surface-enhanced Raman scattering and fluorescence dual-mode aptasensor based on magnetic plasmonic core-shell nanostructures and N-doped carbon dots for aflatoxin B1 detection
Article Snippet: Aflatoxin B1 (AFB1) contamination in foods threatens human health worldwide, and accurate and rapid detection of AFB1 is essential.. Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-mode aptasensor is constructed for sensitive detection of AFB1 based on ZnFe2O4 magnetic nanoparticle@gold nanoparticles-silver shell nanostructures (MNP@AuNPs-Ag) and fluorescent N-doped carbon dots (N-CDs).. Specifically, Raman beacon molecule cyanine 3-modified aptamer (Apt-Cy3) is coupled on the MNP@AuNPs-Ag surface, and complementary DNA (cDNA) is modified on the N-CDs surface (N-CDs-cDNA).
Saline:Article Title: Host-guest confinement in post-metallized COFs for enhanced electrochemiluminescence coupled with Y-shaped DNA amplification for microRNA-499 detection.
Article Snippet: An ultrasensitive electrochemiluminescence (ECL) biosensor was developed for the detection of the acute myocardial infarction (AMI) biomarker miRNA-499 by synergistically integrating an iridium-metallized covalent organic framework (Ir@TpBpy) with enzyme-assisted Y-shaped DNA amplification.. The Ir@TpBpy COF was synthesized via post-metallization of a bipyridine-rich TpBpy framework with Ir(III) complexes.. This design significantly enhances the ECL performance not only through material loading but also via a synergistic optimization strategy: (1) the conjugated framework constructs continuous charge transport pathways to accelerate electron transfer; and (2) the porous architecture induces a guest confinement effect that locally enriches the coreactant triethylamine (TEA), facilitating radical kinetics.
Synthesized:Article Title: Host-guest confinement in post-metallized COFs for enhanced electrochemiluminescence coupled with Y-shaped DNA amplification for microRNA-499 detection.
Article Snippet: An ultrasensitive electrochemiluminescence (ECL) biosensor was developed for the detection of the acute myocardial infarction (AMI) biomarker miRNA-499 by synergistically integrating an iridium-metallized covalent organic framework (Ir@TpBpy) with enzyme-assisted Y-shaped DNA amplification.. The Ir@TpBpy COF was synthesized via post-metallization of a bipyridine-rich TpBpy framework with Ir(III) complexes.. This design significantly enhances the ECL performance not only through material loading but also via a synergistic optimization strategy: (1) the conjugated framework constructs continuous charge transport pathways to accelerate electron transfer; and (2) the porous architecture induces a guest confinement effect that locally enriches the coreactant triethylamine (TEA), facilitating radical kinetics.
Virus:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
Recombinant:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
Polyacrylamide Gel Electrophoresis:Article Title: The genetic repertoire of deep-sea microbiome: From sequence to structure and function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains BL21(DE3) competent cell Vazyme Cat# C504-03 DH5α competent cell Sangon Biotech Cat# B528413-0100 Chemicals, peptides, and recombinant proteins 1M Tris-HCl solution, pH 7.5 Sangon Biotech Cat# B548124 5M sodium chloride solution Sigma-Aldrich Cat# S5150 10X TBE buffer Invitrogen Cat# AM9863 Nuclease-free water Invitrogen Cat# AM9932 HEPES Sigma-Aldrich Cat# 54457 PAGE Gels GenScript Cat# M01012C 20X MOPS/SDS running buffer Sangon Biotech Cat# C506051 30% Acr-Bis (29:1) Beyotime Cat# ST003 0.5M EDTA, pH 8.0 Invitrogen Cat# AM9261 SYBR safe DNA gel stain Invitrogen Cat# S33102 Yeast extract OXOID Cat# LP0021B Tryptone Sangon Biotech Cat# A505250 Imidazole Sangon Biotech Cat# A600277 Manganese(II) chloride tetrahydrate Sigma-Aldrich Cat# M5005 Prestained protein ladder Thermo Fisher Scientific Cat# 26616 Bovine thrombin Yeasen Biotechnology Cat# 20402ES05 BSA Sigma-Aldrish Cat# V900933 DTT Sangon Biotech Cat# A100281 PMSF Sangon Biotech Cat# A100754 Glycerol Sangon Biotech Cat# A100854 DMEM Gibco Cat# 10566016 FBS Gibco Cat# 10091148 DPBS Gibco Cat# 14190144 Tryple Gibco Cat# 12604013 50X TAE Buffer Sangon Biotech Cat# B548101 AO/PI Staining Solution Nexcelom Bioscience Cat# CS2-0106-5ML 10X Loading Buffer TaKaRa Cat# 9157 Acryl/Bis 30% Solution (37.5:1) Sangon Biotech Cat# B546018 10%APS Phygene Cat# PH1808 TEMED Sangon Biotech Cat# A610508 Glycogen, RNA grade Invitrogen Cat# R0551 Agarose, Regular Sangon Biotech Cat# A620014 Recombinant DsCas9 protein, full length, protein ID: DSM_0283490148 This paper N/A Recombinant DSH2 protein, full length, protein ID: DSM_0014922708 This paper N/A Recombinant DSH9 protein, full length, protein ID: DSM_0099674677 This paper N/A Recombinant DSH10 protein, full length, protein ID: DSM_0247050577 This paper N/A Critical commercial assays AMPure XP beads Beckman Coulter Agencourt Cat# B37419AA T4 DNA polymerase Enzymatics Cat# P708L (Continued on next page) Cell Host & Microbe 34, 1–14.e1–e10, August 12, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER TIANamp Genomic DNA kit TIANGEN Cat# DP304 PrimeSTAR® GXL Premix Fast, Dye plus TaKaRa Cat# R052B VAHTS DNA Clean Beads Vazyme Cat# N411-02 T7 Endonuclease I NEB Cat# M0302S Ultra Low Range DNA Ladder Invitrogen Cat# 10597012 NucleoSpin Gel and PCR Clean-up kit MACHERY-NAGEL Cat# 740609.25 T7 High Yield RNA Transcription Kit Vazyme Cat# TR101-01 Guide it IVT RNA Clean Up Kit TaKaRa Cat# 632638 EndoFree Mini Plasmid Kit TIANGEN Cat# DP123 RNeasy PowerFecal Pro Kit QIAGE Cat# 78404 MGIEasy Small RNA Library kit MGI Cat# 940-000196-00 Ni sepharose 6 fast flow Cytiva Cat# 17531804 Single-stranded DNA (ssDNA) cellulose Sigma-Aldrich Cat# D8273 Hitrap desalting columns Cytiva Cat# GE1714-08-01 HiTrap Heparin HP Cytiva Cat# 17-0407-03 HisSep Ni-NTA Agarose Resin Yeasen Biotechnology Cat# 20502ES60 Superdex 200 increase 10/300 GL Cytiva Cat# GE28-9909-44 Nanopore Gene Sequencer MGI Cat# H900-000005-00 DNBSEQ-T7 sequencer MGI Cat# H900-000129-00 CycloneSEQ Universal Library Prep Set MGI Cat# H940-000013 WT Sequencing Flow Cell MGI Cat# H930-000002-00 CycloneSEQ WT Sequencing Kit MGI Cat# H940-000016 Deposited data Raw data, see Table S1.
In Vitro:
Incubation:
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